ABSTRACT
Foram utilizados 159 cavalos Pampa, registrados na Associação Brasileira dos Criadores de Cavalo Pampa, e um grupo-controle, de 32 cavalos da raça Paint, ambos os grupos provenientes de plantéis de diferentes regiões brasileiras, com o objetivo de comparar os testes bioquímico e molecular para detecção de marcadores genéticos para pelagem tobiana em cavalos Pampa. Houve diferença significativa (P<0,001) entre os testes bioquímico e molecular, nos cavalos Pampa, mas o mesmo fato não ocorreu com os da raça Paint. Os resultados mostraram que o marcador molecular (KIT) foi mais eficiente na identificação dos prováveis cavalos homozigotos do que os marcadores bioquímicos albumina (Al) e proteína de ligação da vitamina D (Gc), em ambas as raças.
In this study, 159 Pampa horses, registered at the Associação Brasileira dos Criadores de Cavalo Pampa, and a control group of 32 Paint horses, both coming from herds located in different Brazilian regions, were used to compare biochemical and molecular tests for detection of genetic markers for the Tobiano coat color pattern in Pampa horses. Difference (P<0.001) between biochemical and molecular tess in Pampa horses was observed, but not for the Paint horses. The results showed that the molecular marker (KIT) was more efficient to identify the probable homozygous dominant horses than the biochemical markers albumin (Al) and vitamin D-binding Protein (Gc), in both breeds.
Subject(s)
Animals , Horses/genetics , Immunologic Tests , Homozygote , BiomarkersABSTRACT
Utilizaram-se 195 cavalos Pampa e um grupo-controle de 41 cavalos da raça Paint, provenientes de plantéis de várias regiões brasileiras, com o objetivo de avaliar a eficiência do teste mediante uso de marcadores bioquímicos: albumina (Al) e proteína de ligação da vitamina D (Gc), para identificação dos possíveis indivíduos homozigotos dominantes para o padrão de pelagem tobiano nos cavalos Pampa. Não foram encontrados genótipos AlBB e GcSS, revelando indício de quebra de ligação gênica entre tais locos e o loco tobiano e a ineficácia do teste bioquímico na detecção dos prováveis indivíduos homozigotos dominantes para o padrão de pelagem tobiano nos cavalos Pampa
One hundred and ninety five Pampa horses and a control group of 41 Paint Horses originated from herds located in different Brazilian regions were used to objective of evaluate the efficiency of the biochemical markers albumin (Al) and vitamin D binding protein (Gc) to identify the probable homozygous dominant for the tobiano coat color pattern in Pampa horses. It was not found any genotype AlBB and GcSS, indicating a possible break of the genetic linkage between these loci and the locus Tobiano, as well as the inefficacy of the biochemical test in the detection of the probable homozygous dominant for the tobiano color pattern in Pampa horses
Subject(s)
Animals , Male , Female , Albumins/administration & dosage , Horses/genetics , Genetic Markers/genetics , Vitamin D-Binding Protein/administration & dosageABSTRACT
Foram caracterizados geneticamente, utilizando-se cinco locos de microssatélites, 235 indivíduos de seis plantéis de tilápia (Ceará, Chitralada, Israel, Nilótica, Taiwan e Vermelha) da região Sudeste do Brasil. Verificou-se diferença genética entre os seis plantéis, obtida pelo cálculo do índice de fixação de alelos (Fst=0,3263). De modo geral, está ocorrendo perda de heterozigose nos plantéis, segundo mostrou a estimativa do coeficiente de endogamia intrapopulacional (Fis=0,0486). Os plantéis Israel e Nilótica foram os mais semelhantes geneticamente (Ig=0,6663). Os plantéis Chitralada e Taiwan foram os que menos apresentaram genes em comum (Ig=0,2463). O plantel denominado Vermelha foi o mais distinto entre todos.
Two hundred and thirty five individuals from six commercial stocks of tilapias (Ceará, Chitralada, Israel, Nilótica, Taiwan and Red) from the Southeastern region of the country were genetically characterized using five microsatellite loci. The results suggest large genetic difference among the stocks, estimated through the fixation allele index (Fst = 0.3263), and a considerable loss of heterozigosity accurs in most of the stocks, according to the population inbreeding coefficient (Fis=0.0486). The Israel and Nilótica stocks were genetically similar (Ig=0.6663), while Chitralada and Taiwan showed less genes in common (Ig=0.2463). The Red stock was the most distinct stock.
Subject(s)
Animals , Genetic Variation , Genetic Enhancement/methods , TilapiaABSTRACT
DNA samples of six bovines obtained from three tissues (blood, semen and hair) were extracted using two different techniques. After the extraction procedures the samples were divided in six fractions. Three were stored at -20º C and three at 4º C. Every three months one sample of each tissue/extraction procedure was analyzed in spectrophotometer, to determine the quantity of the DNA and the extract was amplified using the primer RM 29. No differences in the DNA quantity or in the level of protein contamination among the three periods of analyses were observed. All the DNA extracted by quick extraction technique showed good amplification patterns during the nine months, meaning that this technique can be used in laboratory routine instead of the permanent extraction technique. The extract obtained from blood, using the permanent extraction technique, showed the higher quantity of DNA with the smaller index of protein contamination. The high quantity of protein contamination found in the semen samples preserved in egg yolk demanded modifications in both extraction techniques. After that the results were positive, showing good amplification patterns.
Subject(s)
Animals , Blood , DNA , Hair , Molecular Biology , SemenABSTRACT
To evaluate the precision of the DNA tests using the non-automatized technique for individual identification and parentage tests, 105 Rottweiler dogs were studied using the primer CMR S. The sample was composed of 39 animals belonging to 11 complete families and their progenies, and 66 non related individuals until the second generation, derived from kennels located in the states of Minas Gerais and Säo Paulo. The CMR S primer was used for the Polimerase Chain Reaction (PCR). The results showed the inefficiency of the technique, even when analyzed through the automated gel analysis system. Also showed the impossibility of its commercial use due to the fact of does not permit the storage of data for subsequent use
Subject(s)
Deoxyribonucleases , DogsABSTRACT
Foram utilizados 46 animais da raça Gir, registrados na Associaçäo Brasileira de Criadores de Zebu, provenientes de cinco fazendas situadas no Estado de Minas Gerais, com o objetivo de avaliar a eficiência dos microssatélites BM2113, ILSTS005, ILSTS008, ETH131 e RM88 em testes de verificaçäo de parentesco. Os locos BM2113, ILSTS005, ETH131 e RM88 mostraram-se eficientes, apresentando valores de PE2 (probabilidade de exclusäo quando os dois progenitores säo genotipados) entre 0,62 e 0,69 e PIC2 (conteúdo de informaçäo polimórfica quando os dois progenitores säo genotipados) entre 0,78 e 0,83. O mesmo näo ocorreu para o loco ILSTS008, o qual apresentou baixos valores de PE2 (0,24) e PIC2 (0,41)